Automated Cell Volume Estimation in Time-Lapse Microscopy Images
نویسندگان
چکیده
Understanding the genes involved in cell volume regulation is an important area in biological research. Modern screens involving RNA interference often involve analyzing many thousands of cells, and manual techniques are too slow. We describe a fully automated method for estimating the volume of spherical mitotic cells, within given bounds, based on a learned membrane classifier and a circular Hough transform. The method is suited to high-throughput analysis, and its performance is evaluated on a set of manually annotated image sequences.
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